Study of UV-B Mutation Effect on pH Resistance and Lipid Production of Microalgae Botryococcus braunii

Thea Prastiwi Soedarmodjo, Fanina Aulia Rachma, Hakun Wirawista Aparamarta, Arief Widjaja


Microalgae Botryococcus braunii is a potential biodiesel producer as an alternative for fossil fuels due to its high lipid content. UV-B mutations were carried out to see the effect in microalgae growth at various pHs (3-8). Reduction of nitrogen levels was carried out to see the effect on the growth and lipid production of microalgae. UV-B mutation increased the ability of growth and resistance of B. braunii against low pH. Under low nitrogen conditions, the growth of B. braunii cells would not continue for a longer time. B. braunii which grow in nitrogen depletion medium produced lipid content greater than normal nitrogen. UV-B light mutation also increased the lipid content of B. braunii. At 7 days of incubation, the mutation not only increased lipid content, but also significantly increased the TAG content of B. braunii lipids.


Microalgae; Botryococcus braunii; Nitrogen Depletion; pH; mutation; UV-B

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