Ligninolytic Enzymes Produced by Gliomastix sp. in an Organic Waste Medium

Nengah Dwianita Kuswytasari, Maya Shovitri, Enny Zulaika


Ligninolytic enzymes are extracelluler enzymes that have many benefit on multiple industrial department. Its can result from Gliomastix sp. This study was aimed to determine the effect of temperature and pH on the activity of Ligninolytic enzymes by Gliomastix sp. T3.7 that grow on multiple organic waste . Ligninolytic enzymes was measured with spectrofotometer UV-Vis Boeco S22. The result showed that Gliomastix sp. T3.7 potentially produce the ligninolytic enzymes like as laccase, LiP and MnP. The optimum laccase activity ( 1,765 U/ml) detected on sugarcane baggase waste, pH 6 and temperature of 350C . The optimum LiP activity (8,088U/ml) detected on corn cobs, pH 5 and 350C, whereas the optimum MnP activity (0,964 U/ml) detected on sugarcane baggase too. Its optimum on pH 4 and 250C. Gliomastix sp. T3.7 on sugarcane bagasse, pH 6 and 350C, agensia potentially used as remediation on a variety of industry pollution.


Gliomastix sp.; organic waste; ligninolytic enzyme

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S. R. Couto and M. A. Sanromán, "Application of Solid-State Fermentation to ligninolytic Enzyme Production," Biochemical Engineering Journal, vol. 22, no. 3, p. 211–219, 2005.

W.-H. Chen, Y.-J. Tu and H.-K. Sheen, "Disruption of Sugarcane Bagasse Lignocellulosic Structure by Means of Dilute Sulfuric Acid Pretreatment with Microwave-Assisted Heating," Applied Energy, vol. 88, no. 8, p. 2726–2734, 2011.

B. Monties and K. Fukushima, "Occurrence, Function, and Biosynthesis of Lignins," in Biopolymers, Volume 1, Lignin, Humic Substances and Coal, Weinheim, Germany, Wiley- VCH, 2001, p. 1–64.

G. Brunow, "Methods to reveal the structure of lignin," in Biopolymers, Vol. 1—Lignin, Humic Substances and Coal, Weinheim, Germany, Wiley-VCH, 2001, p. 89– 116.

M. Hofrichter, "Review: Lignin Conversion by Manganese Peroxidase (MnP)," Enzyme and Microbial Technology, vol. 30, no. 4, p. 454–466, 2002.

M. Hofrichter, "Review: Lignin Conversion by Manganese Peroxidase (MnP)," Enzyme and Microbial Technology, vol. 30, no. 4, p. 454–466, 2002.

T. K. Kirk and H. M. Chang, Biotechnology in Pulp and Paper Manufacture, New York, USA: Butterworth Hanimenam Publication, 1980.

T. K. Kirk and K. E. Eriksson, "Lignin Biodegradation with white-rot fungi," in Comprehensive Biotechnology Vol. 4, Oxford, JA Pergamon Press Ltd, 1981, pp. 271-294.

M. W. Plat, Y. Hadar, Y. Henis and I. Chet, "Fungal Activities Involved in Lignocellulosic Degradation by Pleurotus sp.," Applied Microbiology and Biotechnology, vol. 20, no. 2, pp. 150-154, 1983.

B. R. M. Vyas, J. Volc and V. Šašek, "Ligninolytic Enzymes of Selected White-Rot Fungi Cultivated on Wheat Straw," Folia Microbiologica, vol. 39, no. 3, pp. 235- 240, 1994.

S. M. Hossain and N. Anantharaman, "Activity Enhancement of Ligninolytic Enzymes of Trametes Versicolor with Bagasse Powder," African Journal of Biotechnology, vol. 5, no. 1, pp. 189-194, 2006.

H. Widiastuti, Suharyanto, A. Wulaningtyas and Sutamihardja, "Activity of Ligninolytic Enzymes during Growth and Fruiting Body Development of White Rot Fungi Omphalina sp. and Pleurotus Ostreatus," HAYATI Journal of Biosciences, vol. 15, no. 4, pp. 140-144, 2008.

R. Bourbonnais and M. Paice, "Oxidation of Non-Phenolic Substrates: An Expanded Role of Laccases in Lignin Biodegration," FEBS letter, vol. 267, no. 1, pp. 99-102, 1990.

M. Tien and T. K. Kirk, "Lignin Peroxidase of Phanerochaete Chrysosporium," Methods in Enzymology, vol. 161, p. 238–249, 1988.

R. I. Syafrizal, Aktifitas enzim Ligninolitik Fungi Pelapuk Putih Omphalina sp. dan Pleurotus Ostreatus pada Limbah Lignoselulosa, Bogor: Program Studi Biokimia, FMIPA, Institut Pertanian Bogor, 2007.

A. Hattaka, "Modifying Enzymes from Selected White-Rot Fungi: Production and Role in Lignin Degradation," FEMS Microbiology Reviews, vol. 13, no. 2-3, pp. 125-135, 1994.

E. E. J. Kaal, J. A. Field and T. W. Joyce, "Increasing Ligninolytic Enzyme Activities in Several White-Rot Basidiomycetes by Nitrogen –Sufficient Media," Bioresource Technology, vol. 53, no. 2, pp. 133-139, 1995.

Y. M. Rohmah, "Studi Potensi Isolat Kapang Tanah dari Wonorejo Surabaya dalam Mendegradasi Lignin," Biologi ITS, Surabaya, 2012.

S. Sen, P. P. Singh, V. S. Rathore and B. M. J. Pereira, "Sugarcane Bagasse Improves The Activity of Ligninolytic Enzymes and Decolourization of Dyes by The White-Rot Fungus Pleurotus Ostreatus," Journal of Scientific & Industrial Research, vol. 63, no. 9, pp. 739-746, 2004.

H. M. Cunha and F. T. Silva, "Identification of Low Molecular Weight Aromatic Compounds Present in The Hydrolysate Obtained From Sugarcane Baggase Pretreated by Steam Explosion," in 6th Brazilian Symposium Chemistry of Lignin and Other Wood Components, Guaratingueta, Sao paulo, Brazil, 2001.

D. S. Arora and P. K. Gill, "Effects of Various Media and Supplements on Laccase Production by some White Rot Fungi," Bioresource Technology, vol. 77, no. 1, pp. 89-91, 2001.

C. Raghukumar, T. M. D'souza, R. G. Thorn and C. A. Reddy, "Lignin-Modifying Enzymes of Flavodon Flavus, Basidiomycete Isolated from A Costal Marine Environment," Applied and Environmental Microbiology, vol. 65, no. 5, pp. 2103-2111, 1999.

J. E. Bailey and D. F. Ollis, Biochemical Engineering Fundamentals, New York: McGraw-Hill Inc, 1981.

M. L. Shulter and F. Kargi, Bioprocess engineering : Basic concept, New Delhi: Parentice-Hall of India Pvt Ltd, 2000.

M. J. Pelezar, E. C. S. Chan and N. R. Kring, Microbiology, 5th edition, New Delhi: Tata McGraw-Hill Publishing Co Ltd, 2004.

F. Sun, J. Li, Y. Yuan, Z. Y. Yan and X. F. Liu, "Effect of Biological Pretreatment with Trametes Hirsuta yj9 on Enzymatic Hydrolysis of Corn Stover," International Biodeterioration & Biodegradation, vol. 65, no. 7, pp. 931-938, 2011



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