Epidemiology and Characteristics of Resistant Typhoidal Salmonella Strains Prevalent in Lafia, Nigeria

Jebes Lamini Ngolo, Olukayode Orole, Aleruchi Chuku

Abstract


Resistance to antibiotics by pathogens is a burden to disease management. The study characterized Salmonella species in patients with fever and determined their antibiotic susceptibility patterns in Dalhatu Araf Specialist Hospital Lafia, Nigeria. Faecal samples were collected from 400 patients, and Salmonella species were isolated. The isolates were rested against ten antibiotics to determine their multi-drug and extensively drug-resistant capabilities, after which molecular characterization was done. Male patients between the ages of 21 to 30 years had the highest prevalence of 17(44.7%) of the pathogen, and they were the most susceptible among those who regularly drank water from boreholes (19(50.0%)). Augmentin with 44(73.0%) Salmonella susceptibility was the most effective antibiotic, while gentamycin, ampicillin, chloramphenicol, and cotrimoxazole had the isolates obtaining resistance of 60(100%), 59(98.0%), 57(95.0%), and 56(93.4%) respectively. Only six of the 60 Salmonella isolates were not multidrug-resistant, while 25 were extensively drugresistant. Isolates S9 and S25 with staG genes were the only Salmonella typhi found in the study; others with ttr genes were of the Salmonella genera. The study concludes that the first-line antibiotics administered to patients with typhoid fever are no longer effective; hence, proactive measures should be put in place to surmount the observed challenge.

Keywords


Antibiotics; Lafia; Pathogen; Prevalence; Resistance; Typhoidal Salmonela

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References


Giri S, Mohan VR, Srinivasan M, Kumar N, Kumar V, Dhanapal P, et al. Case-Control Study of Household and Environmental Transmission of Typhoid Fever in India. Journal of Infectious Diseases 2021;224:584–592.

Nair S, Day M, Godbole G, Saluja T, Langridge GC, Dallman TJ, et al. Genomic surveillance detects Salmonella enterica serovar Paratyphi A harbouring blaCTX-M-15 from a traveller returning from Bangladesh. PLoS ONE 2020;15(1):e0228250.

Andino A, Hanning I. Salmonella enterica: Survival, colonization, and virulence differences among serovars. Scientific World Journal 2015;2015:1–16.

Bell BG, Schellevis F, Stobberingh E, Goossens H, Pringle M. A systematic review and meta-analysis of the effects of antibiotic consumption on antibiotic resistance. BMC Infectious Diseases 2014;14(13):1–25.

Sheshe A, Anyanwu LJ, Mohammad A, Muhammad A, Obaro S. Typhoid intestinal perforation: Analysis of the outcome of surgical treatment in Kano, Nigeria. Archives of Medicine and Health Sciences 2018;6(1):59.

Olowo-okere A, Ibrahim YKE, Olayinka BO. Molecular characterisation of extended-spectrum β-lactamase-producing Gram-negative bacterial isolates from surgical wounds of patients at a hospital in North Central Nigeria. Journal of Global Antimicrobial Resistance 2018 9;14:85–89.

Ye Q, Wu Q, Zhang S, Zhang J, Yang G, Wang J, et al. Characterization of extended-spectrum β-lactamase-producing Enterobacteriaceae from retail food in China. Frontiers in Microbiology 2018;9(AUG):1–12.

Liilian A, Graba S, Moses A. Sero Prevalence of Salmonella typhi among Pregnant Women in Niger State. Journal of Microbiology 2015;(3):118–121.

Akinyemi KO, Iwalokun BA, Alafe OO, Mudashiru SA, Fakorede C. BlaCTX-M-I group extended spectrum beta lactamaseproducing Salmonella typhi from hospitalized patients in Lagos, Nigeria. Infection and Drug Resistance 2015;8:99–106.

Abdullahi M. Incidence and antimicrobial susceptibility pattern of salmonella species in children attending some hospitals in kano metropolis, kano state –Nigeria. Bayero Journal of Pure and Applied Sciences 2010;3(1):27–32.

Lamini JN, Nfongeh JF, Orole OO. Extended Beta-Lactamase (ESBL) Producing Salmonella typhi from Presumptive Typhoid Patients in Nasarawa State, Nigeria. Greener Journal of Epidemiology and Public Health 2018 6;6(3):80–86.

Grema BA, Aliyu I, Michael GC, Musa A, Fikin AG, Abubakar BM, et al. Typhoid ileal perforation in a semi-urban tertiary health institution in north-eastern Nigeria. South African Family Practice 2018;60(5):168–173.

Zhang S, Yin Y, Jones MB, Zhang Z, Kaiser BLD, Dinsmore BA, et al. Salmonella serotype determination utilizing high-throughput genome sequencing data. Journal of Clinical Microbiology 2015;53(5):1685–1692.

Gymoese P, Sørensen G, Litrup E, Olsen JE, Nielsen EM, Torpdahl M. Investigation of outbreaks of Salmonella enterica serovar typhimurium and its monophasic variants using whole-genome sequencing, Denmark. Emerging Infectious Diseases 2017;23(10):1631–1639.

Alvarez-Uria G, Gandra S, Mandal S, Laxminarayan R. Global forecast of antimicrobial resistance in invasive isolates of Escherichia coli and Klebsiella pneumoniae. International Journal of Infectious Diseases 2018;68:50–53.

Clinical C, Institute LS. Performance Standards for Antimicrobial Susceptibility Testing. 31 ed. Clinical and Laboratory Standards Institute; 2017.

Klemm EJ, Shakoor S, Page AJ, Qamar FN, Judge K, Saeed DK, et al. Emergence of an extensively drug-resistant Salmonella enterica serovar typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third-generation cephalosporins. mBio 2018;9(1):1–10.

Tsaku PA, Ngwai YB, Pennap GRI, Ishaleku D, Ibrahim T, Nkene IH, et al. Extended-Spectrum BetaLactamase–production in Escherichia coli isolated from door handles in Nasarawa State University, Keffi, Nigeria. Heliyon 2019;5(8):E02177.

Nair S, Patel V, Hickey T, Maguire C, Greig DR, Lee W, et al. Real-time PCR assay for differentiation of typhoidal and nontyphoidal Salmonella. Journal of Clinical Microbiology 2019;57(8):e00167–19. https://journals.asm.org/doi/10.1128/JCM.00167-19.

Guard-Petter J. The chicken, the egg and Salmonella enteritidis. Environmental Microbiology 2001;3(7):421–430. https://ami-journals.onlinelibrary.wiley.com/doi/abs/10.1046/j.1462-2920.2001.00213.x.

Crump JA. Progress in Typhoid Fever Epidemiology. Clinical Infectious Diseases 2019 02;68(1):S4–S9. https://doi.org/10.1093/cid/ciy846.

Okpa BO, Gberikon GM, Oranusi S, Ichor T. Isolation and Molecular Characterization of Salmonella Serovars Distributed in Benue State, Nigeria. In: Green Energy and Technology Springer; 2022.p. 317–330.

E Asikong Ernest B, Aleruchi C, Reuben Tiku D, Godwin O, Vivian A. Comparative Study of Bacteriological Quality of NAFDAC Registered and Unregistered Sachet Water Sold in Lafia Metropolis. Journal of Advances in Biology & Biotechnology 2016 12;10(4):1–9. https://journaljabb.com/index.php/JABB/article/view/176.

Kim C, Latif I, Neupane DP, Lee GY, Kwon RS, Batool A, et al. The molecular basis of extensively drug-resistant Salmonella Typhi isolates from pediatric septicemia patients. PLoS ONE 2021;16(9):1–15.

Mutai WC, Muigai AWT, Waiyaki P, Kariuki S. Multi-drug resistant Salmonella enterica serovar Typhi isolates with reduced susceptibility to ciprofloxacin in Kenya. BMC Microbiology 2018;18(1):1–5.

Chiou CS, Lauderdale TL, Phung DC, Watanabe H, Kuo JC, Wang PJ, et al. Antimicrobial resistance in Salmonella enterica serovar Typhi isolates from Bangladesh, Indonesia, Taiwan, and Vietnam. Antimicrobial Agents and Chemotherapy 2014;58(11):6501–6507.

Sharma A, Sharma R, Sharma S, Sharma A, Soni D. Typhoid intestinal perforation: 24 Perforations in one patient. Annals of Medical and Health Sciences Research 2013;3(5):41.

System NARM. NARMS Integrated Report 2014; 2016.

Penesyan A, Gillings M, Paulsen IT. Antibiotic discovery: Combatting bacterial resistance in cells and in biofilm communities. Molecules 2015;20(4).

Stanaway JD, Reiner RC, Blacker BF, Goldberg EM, Khalil IA, Troeger CE, et al. The global burden of typhoid and paratyphoid fevers: a systematic analysis for the Global Burden of Disease Study 2017. The Lancet Infectious Diseases 2019;19(4):369–381.

Adedokun AA, Orevaoghene OE, Abah AE. Intestinal Parasites and Salmonella typhi Infection among Food-handlers in Port Harcourt Metropolis, Nigeria. Journal of Advances in Medicine and Medical Research 2020;p. 1–9.

Agbulu EU. Distribution Pattern Of Salmonella Typhoidal Serotypes In Benue State Central, Nigeria. The Internet Journal of Epidemiology 2012;8(1):1–7.

Chirambo AC, Nyirenda TS, Jambo N, Msefula C, Kamng’ona A, Molina S, et al. Performance of molecular methods for the detection of Salmonella in human stool specimens. Wellcome Open Research 2021;5:237.




DOI: http://dx.doi.org/10.12962/j20882033.v34i1.14677

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