Analysis of N-nitrosodiprophylamines Carcinogenic Compound in Meat-Processing using Headspace-Single Drop Microextraction-Gas Chromatography-Flame Ionization Detector (HS-SDME-GC-FID)

Teguh Hari Sucipto, Ganden Supriyanto, Yanuardi Raharjo

Abstract


Analysis of N-nitrosodiprophylamines carcinogenic compound in processed meat especially hamburger and kebab had occured by HS-SDME-GC-FID technique. The results were obtained determining the optimum pH was 4, the optimum stirring speed was 6 scale, and the temperature of extraction was 30 ºC. It was obtained in this study that the detection limit of 78 ppb, the percent recovery of 101,18%, precision between 0,089% to 0,566%, and the true enrichment factor was 3372,66 times. From the results of the study was concluded that HS-SDME-GC-FID technique can be used to analyze the carcinogenic compound N-nitrosodiprophylamines (NDPA) found in meat-processing (hamburger and kebab) by the concentration of each samples as follows, hamburger I of 0,27 ppm, hamburger II of 0,73 ppm, hamburger III of 1,39 ppm, and kebab I of 3,13 ppm

Keywords


HS-SDME-GC-FID technique; N-nitrosodiprophylamines; Meat-processing

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References


Domanska-Blicharz, K., Rachubik J., Kowalski, B., 2005, Occurrence of Volatile N-Nitrosamines in Polish Tinned Foods, Bull Vet Inst Pulawy, 49, 319-322.

Andrade, R., Reyes, F.G.R., Rath S., 2005, A Method For The Determination of Volatile N-Nitrosamine in Food by HS-SPME-GC-TEA, J. Food Chem., 91: 173-179.

Filho, P.J.S., Rios, A., Valcárcel, M., Zanin, K.D., Caramão, E.B., 2003, Development of a New Method for The Determination of Nitrosamines by Miceller Electrokinetic Capillary Chromatography, Water Research, 37: 3837-3842.

Riccio, D., Wood, D.C., Miller, J.M., 2008, Using Single Drop Microextraction for Headspace Analysis with Gas Chromatography, J. of Chem. Education, 85(7), 965-968.

Sleiman, M., Maddalena, R.L., Gundel, L.A., dan Destaillats, H., 2009, Rapid and Sensitive Gas Chromatography-Ion-Trap Tandem Mass Spectrometry Method for The Determination of Tobacco-Specific N-Nitrosamines in Secondhand Smoke, J. of Chrom. A, 1216, 7899-7905.

Miller, J.C., dan Miller, J.N., 1988, Statistics for Analytical Chemistry Second Edition, Elllis Horwood Limited, England.




DOI: http://dx.doi.org/10.12962/j23546026.y2015i1.1128

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