Subcritical Water Extraction of Phenolic Compounds from Moringa Oleifera Leaf

Siti Zullaikah, Irfan Saputra, Ghuzrina Prihandini, M. Rachimoellah

Abstract


Moringa oleifera leaf is a good source of phenolic compounds that are reported to exhibit antioxidant activity both in vitro and in vivo. This study investigated the extraction of phenolic compounds from Moringa oleifera leaf using subcritical water. Experiments were performed in a batch stainless steel reactor at temperature ranging from 100 to 300oC at residence time of 5 to 20 min. Subcritical water extraction resulted the highest yield of product, total phenolic compounds and antioxidant activity at temperature of 200oC at residence time of 15 minutes. The yield of product, total phenolic compounds and antioxidant activity obtained were 30.661%, 48.733 mg tannat acids/ g dry powder of extract and 45.863 mg ascorbic acid/L, respectively. Subcritical water at 200°C and 15 min might be a good substitute to organic solvents such as ethanol to obtain phenolic compounds from Moringa oleifera leaf.

Keywords


antioxidant; hydrothermal; Moringa oleifera leaf; phenolic; subcritical water

Full Text:

PDF

References


. Aqil, F., Ahmad, I., & Mehmood, Z. 2006. Antioxidant and free radical scavenging properties of twelve tradisional used Indian medicinal plants. In: Moyo, B., Oyedemi, S. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Turkish journal of Biology, 30, 177-183.

. Okwu, D.E. 2004. Phytochemicals and vitamin content of indigenous spesies of South Eastern Nigeria. Journal of Sustainable Agriculture and Environment, 6, 30-34.

. Zheng, R.L., Huang, Z.Y., 2001. Reactive oxygen spesies. In: Zheng, R.L., Huang, Z.Y. (Eds.), Free Radical and Agricultural Science. China Higher Education Press and Springer Press, Beijing, pp. 17-27.

. Peng, Z.P., Strack, D., Baumert, A., Subramaniam, R., Goh, N.K., Chia, T.F., Tan, S.N., and Chia, L.S. 2003. Antioxidant Flavonoids from leaves of Polygonum hydropiper L. Journal of Phytochemistry In Elsevier Science Ltd,62, 219-228.

. Siddhuraju, P., & Becker , K.2003. Antioxidant Properties of various solvent extracts of total phenolics constituens from three different agroclimatic origins of drumstick tree (Moringa oleifera lam) leaves. In :Moyo, B., Oyedemi, S. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Journal of Agricultural and Food Chemistry, 51, 2144-2155.

. Nadkarni, A.K. 1976. Indian Materia Medica. Popular Prakashan, Bombay, pp. 810-816.

. Parrotta, J.A. 2001. Healing Plants of Peninsular India. CABI Publication, pp. 528-530.

. Rao, A.V., Devi, P.U., and Kamath, R. 2001. In vitro radioprotective effect of Moringa oleifera leaves. Indian J. Exp. Biol. 39, 858-863.

. Murakami, A., Kitazono, Y., Jiwajinda, S., Koshimizu, K., Ohigashi, H. 1998. Niaziminin, a thiocarbonate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein-Barr virus activation. Planta Medicines,64, 319-323.

. Faizi, S., Siddiqui, B.S., Saleem, R., Shahreen, F., Gilani, A.H. 1998. Hypotensive constituents from the pods of Moringa oleifera. Planta Medicine, 64, 225-228.

. Bharli, R.,Tabassum, J., and Azad M.R.H. 2003. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolisir enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pacific J Cancer Prev, 4, 131-139.

. Guevara, A.P., and Vargas, C. 1999. An antitumor promoter from Moringa oleifera Lam. Mutat. Res. 440, 181-188.

. Ezeamuzle, I.C., Ambadederomo, A.W., Shode, F.O., Ekwebelem, S.C. 1996. Anti-inflammatory effects of Moringa oleifera root extract. International. J. Pharmacogn, 34, 207-212.

. Ruckmani, K., Kavimani, S., Anandan, R., Jaykar, B. 1998. Effect of Moringa oleifera Lam on paracetamol-induced hepatoxicity. Indian J. Pharm. Sci, 60, 33-35.

. Herrero, M., Cifuentes, A., Ibanez, E. 2006. Subcritical and Supercritical fluida extraction of functional ingredients from different natural sources; plants, food-by-products, alga and microalgae. Food Chemicals, 98, 136-148.

. Ramos, L., Kristenson, E.M., Brinkman, U.A.T., 2002. Current used of pressurised liquid extraction and subcritical water extraction in environmental analysis. Journal of Chromatography, A 975, 3-29.

. King, J. 2003. Development and potential of critical fluida Technology in the nutraceutical industry. In: York, P., Kompella, U.B., Shekunov, B.V. (Eds), Drug delivery and Supercritical Fluid Technology. Marcel Dekker, New York, 579-614.

. Andersson, T.,2007. Parameters affecting the extraction of Polycyclic aromatik hydrocarbons with pressurised hot water. Academic dessertation, University of Helsinki. Finland

. Vongsak, B., Sithisarn, P., Mangmool, S., Thongpraditchote, S., Wongkrajang, Y., and Gritsanapan, W. 2012. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Journal of Crops and Products, xxx, xxx-xxx.

. Wang, L., Weller, C.L. 2006. Recent Advances in extraction of nutraceuticals from plants. Trends Food Science Technology, 17, 300-312,

. Wiboonsirikul, J., Kimura, Y., Kadota, M., Morita, H., Tsuno, T., Adachi, S. 2007. Properties of extracts from defatted rice bran by its subcritical water treatment. Journal of Agricultural in Food Chemicals, 55, 8759-8765.

. Luque de Castro, M.D., Jiminez-Carmona, M.M., Perez, Fernandez., 1999. Towards more rational technique for the isolation of valuable essential oils from plants. Trends Analytical Chemical, 18, 708-716.

. Hatta, S., Wiboonsirikul, J.,Maeda, A., Kimura, Y., Adachi, S.2008. Extraction of defatted rice bran by subcritical water treatment. Biochemical Engineering Jpurnal, 40, 44-53.

. Ko, M.J., Cheigh, C.I., Cho, S.W., Chung, M.S.2010. Subcritical water extraction of flavonol quercetin from onion skin. Journal of Food Engineering, 102, 327-333.

. Pothitirat, W., Chomnawang, M.T., Supabphol, R., Gritsanapan, W., 2009. Comparison of bioactive compound content, free radical scavenging and anti-acne inducing bacteria activities of extract from the mangosteen fruit rind at two stages of maturity. Fitoterapia, 80, 442-447.

. Ramos, L., Kristenson, E. M., & Brinkman, U. A. (2002). Current use of pressurized liquid extraction and subcritical water extraction in environmental analysis. Journal of Chromatography A, 975, 3−29

. Kubatova, A., Lagadec, A.J.M., Miller, D.J., Hawthorne, S.B., 2001. Selective extraction of oxygenates from savory and peppermint using subcritical water. Flavour and Fragrance Journal 16, 64–73.




DOI: http://dx.doi.org/10.12962/j23546026.y2014i1.397

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.