Isolation and antidiabetic Activity of Prenylated Xanthones from Pericarp of Mangosteen (Garacinia Mangostana Linn.)

Syahdam Karneng, Arif Fadlan, Taslim Ersam

Abstract


The material used in this research was dry powder of pericarp mangosteen (Garcinia mangostana Linn.). which is a family plant of Clusiaceae. This taxa is known as the main source of prenylated xanthones derived from phenolic compounds. The compounds separation process was carried out by maceration method, 8 kg of sample macerated using n-hexane solvent, 90 g of n-hexane extract were produced. The n-hexane extract was fractionated by vacuum liquid chromatography (VLC) using silica gel, eluted by increasing the polarity of the solvent, which is a mixture of n-hexane solvent: dichloromethane (10%, 20%, 30% and 50%), resulting in three combined fractions, namely the first fraction 13,92 g, the second fraction 18,27 g, and the third fraction 23,24 g. The refraction process was then carried out in the third fraction, producing two yellow crystals with melting points respectively, 155-156 °C and 172-173 °C. The structural elucidation method was carried out using UV-Vis, IR, HR-ESI-MS, and NMR spectroscopy techniques. Based on the analysis of spectrum data from two known compounds, 8-deoxygartanin (1) and β-mangostin (2) antidiabetic bioactivity test was carried out by the method of inhibiting the enzyme α-glucosidase in vitro. The test results obtained by compound (1) with an IC50 value of 38,5 μM and compound (2) with an IC50 value of 157,9 μM indicate that the two compounds are included in the inactive category. This is indicated by the IC50 value which is much higher when compared to the acarbose as a positive control with an IC50 value of 4.5 μM.

Keywords


Garcinia mangostana Linn; Isolation; Antidiabetic.

Full Text:

PDF

References


Hidayat, J. S. (2018). Delivering The Vision-Laporan Tahunan. Jakarta: PT. Sido Muncul.

Santoso, B., Rifqi, S. U., Muhammad, D. W. (2016). Analisis Hubungan Senyawa Golongan Flavonoid Dari 24 Famili Tanaman Terhadap Aktivitas Penangkap Radikalnya. Prosiding Seminar Nasional Kimia F.Farmasi. Surakarta: Universitas Muhammadiyah Surakarta.

Pedraza-Chaverri, J., Noemi, C-R., Marisol, O-I., Jazmin, M. P-R. (2008). Review: Medicinal Properties of Mangosteen (Garcinia Mangostana L.). Food and Chemical Toxicology, 46(10), 3227–3239.

Nofriati, D., Dewi, N., Herwenita. (2019). Prospek Pemanfaatan Kulit Buah Manggis Menjadi Minuman Herbal Sebagai Upaya Meningkatkan Nilai Tambah Produk (Studi Kasus Di Provinsi Jambi). In PNK BPTP Jambi, Peneliti BPTP Jambi, PNK BPTP Sum Sel.

Pagare, S., Manila, B., Niraj, T., Sonal, P., Y. K., Bansal. (2015). Secondary Metabolites of Plants and Their Role: Overview. Current Trends in Biotechnology and Pharmacy Journal, 9(3), 293–304.

Tiwari, R., C. S. Rana. (2015). A Review: Plant secondary metabolites. International Journal of Engineering Research and General Science, 3(5), 153–159.

Obolskiy, D., Ivo, P., Nisarat, S., Michael, H. (2009), Review : Garcinia mangostana L.: a Phytochemical and Pharmacological. Phytotherapy Research. Vol. 23, No. 8, 1047–1065.

Fatmawati, S., Kuniyoshi, S., Ryuichiro, K. (2011). Ganoderol B: A Potent Glucosidase Inhibitor Isolated From The Fruiting Body of Ganoderma Lucidum. Phytomedicine Journal, 18(12), 1053–1055.

Jung, H. A., Bao, N. S., William, J. K., Rajendra, G. M., A. Doughlas, K. (2006). Antioxidant Xanthones From The Pericarp of Garcinia mangostana (Mangosteen). Journal of Agricultural and Food Chemistry, 54(6), 2077–2082.

Wulandari, A. W. (2017). Isolasi Dua Senyawa Santon Dari Ekstrak Batang Metilen Klorida Garcinia tetrandra Pierre. Skripsi pada FMIPA. Surabaya: Institut Teknologi Sepuluh Nopember.

Jayanti, H. S. D. (2018). Digeranilasi Santon Pada Ekstrak Diklorometana

Kulit Batang Wadung (Garcinia tetranda Pierre). Skripsi pada FIA. Surabaya: Institut Teknologi Sepuluh Nopember.

Ibrahim, S. R. M., Gamal, A. M., Maan, T. K., Sahar, A., Hany, A-H., Khalid, Z, A. (2019). Mangostanaxanthone Viiii, A New Xanthone From Garcinia mangostana Pericarps, α-Amylase Inhibitory Activity, and Molecular Docking Studies. Revista Brasileira de Farmacognosia Journal, 29(2), 206–212.

Nguyen, L-H, D., Hau, T, V., Hung, D, P., Joseph, D, C., Leslie, J, H. (2012). Xanthones From The Bark of Garcinia pedunculata. Phytochemistry Letters, 5(4), 766–769.

Lukis, P.A. (2011). Dua Senyawa Mangostin Dari Ekstrak n-Heksana Pada Kayu Akar Manggis (Garcinia mangostana Linn.) Asal Kab. Nganjuk, Jawa Timur. In Prosiding Kimia FMIPA. Surabaya: Institut Teknologi Sepuluh Nopember.




DOI: http://dx.doi.org/10.12962/j23546026.y2020i6.8940

Refbacks

  • There are currently no refbacks.


View my Stat: Click Here

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.