Bioactive Compounds and Antioxidant Evaluation of Methanolic Extract of Hibiscus Sabdariffa

Stephen Adusei

Abstract


Hibiscus Sabdariffa (Roselle) is renowned for its delicacy and healing properties. Thestudy aimed at evaluating the bioactive compounds (phytochemicals) and antioxidantactivity of leaf, stem, and calyx of roselle, extracted with methanol. Phytochemicalswere identified using numerous test methods and quantified with a UV-Vis spec-trophotometer. The screening test revealed a higher number of phytochemicals in thecalyx extract than the leaf or stem. With the quantification, the highest concentrationwas recorded in the calyx, followed by the leaf and then the stem. The antioxi-dant activity of the investigated plant was in the order of calyx>leaf>stem. Higherrecoveries, ranging between 90%-110%, was obtained for all compounds. Therefore,this study provides evidence that the leaf, stem, and calyces of roselle plants arethe probable source of antioxidants and many bioactive compounds, justifying theirethnopharmacological use in conventional medicines.

Keywords


Antioxidant Evaluation; Bioactive Compounds; Hibiscus Sabdariffa; UV-Vis Spectrophotometer

Full Text:

Full Text

References


O B, OO A. Microbiological and Physico-chemical Evaluation of some Non-alcoholic Beverages. Pakistan Journal of Nutrition 2004;3:188–192.

Akindahunsi A, Olaleye M. Toxicological investigation of aqueous-methanolic extract of the calyces of Hibiscus sabdariffa L. Journal of ethnopharmacology 2003;89(1):161–164.

BH A, Al W, G B. Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L. Phytotherapy research 2008;19(5):369–375.

IS O, OE N, UB OU. Changes in the quality of Zobo beverages produced from Hibiscus sabdariffa (Linn Roselle) and the effects of extract of ginger alone or in combination with refrigeration. African Journal of Biotechnology 2007;7(8):1176–1180.

y Ortiz C M, P CE. Plantas Medicinales Utilizadas en el Estado de Morelos. 2 ed. CCNABIO; 2007.

Osueke J, Ehirim F. Chemical, nutritional and sensory analysis of zobo drink (var sabdariffa) and selected soft drinks. Journal of Agriculture and Food Sciences 2004;2(1):21–24.

Thakuria P, Nath Professor R, Sarma Professor S, Kalita Professor D, Dutta Professor D, Borah Professor P, et al. Phytochemical screening of medicinal plants occurring in local area of Assam. Journal of Pharmacognosy and Phytochemistry JPP 2018;186(73).146 Adusei

SN K, S T, AO J. Studies on Bioactive Saponins from Chinese Medicinal Plants. Advances in Experimental Medicine and Biology 2006;404:371–382.

Koche D, Shirsat R, Kawale M. An overerview of major classes of phytochemicals: their types and role in disease prevention. Hislopia Journal 2016;9:1–11.

Suffredini I, Sader H, Goncalves A. Screening of antibacterial extracts from plants native to the Brazilian amazon rain forest and Atlantic forest. Braz J Med Biol Res 2004;37:379–384.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants 2017;6(4):42.

Ncube N, Afolayan A, Okoh A. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African journal of biotechnology 2008;7(12):1797–1806.

Trease G, Evans W. Pharmacognosy. 2002. 15 ed. Harbcourt publishers Ltd., WB Saunders Company Ltd; 2002.

Cannel R. Methods in Biotechnology. Natural Products Isolation. Human press, New Jersey; 2000.

Adegoke A, Iberi P, Akinpelu D, Aiyegoro O, Mboto C. Studies on phytochemical screening and antimicrobial potentials of Phyllanthus amarus against multiple antibiotic resistant bacteria. International Journal of Applied Research in Natural Products 2010;3(3):6–12.

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin– Ciocalteu reagent. Nature protocols 2007;2(4):875–877.

C P, P M, W N. Antioxidant activities and phenolic contents of extracts from Salvinia molesta and Eichornia Cressipes. Res J Biol Sci 2009;4:1113–1117.

F M, M K, N G, HR V. Phytochemical screening of some species of Iranian plants. Iranian Journal of Pharmaceutical Resource 2003;5:77–82.

Adusei S, Otchere JK, Oteng P, Mensah RQ, TeiMensah E. Phytochemical analysis, antioxidant and metal chelating capacity of Tetrapleura tetraptera. Heliyon 2019;5(11):e02762.

Akinmoladun AC, Ibukun E, Afor E, Obuotor EM, Farombi E. Phytochemical constituent and antioxidant activity of extractfrom the leaves of Ocimum gratissimum. Scientific Research and Essays 2007;2(5):163–166.

Koleva II, Van Beek TA, Linssen JP, Groot Ad, Evstatieva LN. Screening of plant extracts for antioxidant activity: acomparative study on three testing methods. Phytochemical Analysis: An International Journal of Plant Chemical andBiochemical Techniques 2002;13(1):8–17.

Melo EdA, Maciel MIS, Lima VLAG, Leal FLL, Caetano ACdS, Nascimento RJ. Antioxidant capacity of vegetablescommonly consumed. Food Science and Technology 2006;26(3):639–644.

M W, T S, B LB. Modes of action of allele chemical alkaloids: interaction with neuro receptors, DNA and other moleculartargets. Journal of chemical ecology 2010;24:1888–1937.

Silva E, Souza J, Rogez H, Rees JF, Larondelle Y. Antioxidant activities and polyphenolic contents of fifteen selected plantspecies from the Amazonian region. Food chemistry 2007;101(3):1012–1018.

Tapas AR, Sakarkar D, Kakde R. Flavonoids as nutraceuticals: a review. Tropical journal of Pharmaceutical research2008;7(3):1089–1099.

Traore F, Faure R, Ollivier E, Gasquet M, Azas N, Debrauwer L, et al. Structure and antiprotozoal activity of triterpenoidsaponins from Glinus oppositifolius. Planta Medica 2000;66(4):368–371.

Formagio A, Ramos D, Vieira M, Ramalho S, Silva M, Zárate N, et al. Phenolic compounds of Hibiscus sabdariffa andinfluence of organic residues on its antioxidant and antitumoral properties. Brazilian Journal of Biology 2015;75(1):69–76.

Tsai PJ, McIntosh J, Pearce P, Camden B, Jordan BR. Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffaL.) extract. Food research international 2002;35(4):351–356.

Lasker YB, Mazumder PB. Insight into the molecular evidence supporting the remarkable chemotherapeutic potential ofHibiscus sabdariffa L. Biomedicine and Pharmacotherapy 2020;127:110153.




DOI: http://dx.doi.org/10.12962/j20882033.v31i2.6572

Refbacks

  • There are currently no refbacks.


Creative Commons License

IPTEK Journal of Science and Technology by Lembaga Penelitian dan Pengabdian kepada Masyarakat, ITS is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Based on a work at https://iptek.its.ac.id/index.php/jts.