Effect of Dilute Acid Pretreatment of Vegetable Waste on Sugar Production and Inhibitor Formation

Denistira Fazlur Rahman, Hakun Wirawasista Aparamarta, Arief Widjaja

Abstract


Vegetable waste is an organic waste with high cellulose and hemicellulose and low lignin content. The cellulose and hemicellulose chains can be broken down by pretreatment using sulfuric acid to obtain reducing sugar. To avoid the formation of degradation products that have the potential as inhibitor compounds, the temperature of the pretreatment operation was carried out at 121 and 125 for 60 minutes with sulfuric acid concentrations varying from 0.5% to 1.5% (v/v). The solid and liquid ratio (S/L) was 5% (w/v). From the experiments, the highest total reducing sugars of 7.068 g/L was obtained by pretreatment conditions at 121 for 60 minutes with a sulfuric acid concentration of 1% (v/v). Meanwhile, the lowest total reducing sugar of 2.764 g/L was produced during the pretreatment operating conditions at 125 for 60 minutes with a sulfuric acid concentration of 1% (v/v). Under the present experimental condition, it was found that only a low level of degradation product was formed, which ensures excellent performance of bacterial growth in the subsequent fermentation process.

Keywords


Dilute Sulfuric Acid; Inhibitor Compounds; Sugar Production; Vegetable Waste

Full Text:

Full Text

References


Saidi R, Liebgott PP, Hamdi M, Auria R, Bouallagui H. Enhancement of Fermentative Hydrogen Production by Thermotoga Maritima Through Hyperthermophilic Anaerobic Co-Digestion of Fruit-Vegetable and Fish Wastes. International Journal of Hydrogen Energy 2018;43(52):23168–23177.

Chookaew T, O-thong S, Prasertsan P. Biohydrogen Production from Crude Glycerol by Two Stage of Dark and Photo Fermentation. International Journal of Hydrogen Energy 2015;40(24):7433–7438.

Singh A, Kuila A, Adak S, Bishai M, Banerjee R. Utilization of Vegetable Wastes for Bioenergy Generation. Agricultural Research 2012;1(3):213–222.

Kapoor M, Raj T, Vijayaraj M, Chopra A, Gupta RP, Tuli DK, et al. Structural Features of Dilute Acid, Steam Exploded, and Alkali Pretreated Mustard Stalk and Their Impact on Enzymatic Hydrolysis. Carbohydrate Polymers 2015;124:265–273. http://dx.doi.org/10.1016/j.carbpol.2015.02.044.

Gonzales RR, Sivagurunathan P, Parthiban A, Kim SH. Optimization of Substrate Concentration of Dilute Acid Hydrolyzate of lignocellulosic Biomass in Batch Hydrogen Production. International Biodeterioration and Biodegradation 2016;113:22–27.

Rolly R, Kumar G, Sivagurunathan P, Kim Sh. Enhancement of Hydrogen Production by Optimization of pH Adjustment and Separation Conditions Following Dilute Acid Pretreatment of Lignocellulosic Biomass. International Journal of Hydrogen Energy 2017;42(45):27502–27511.

Park JH, Cheon HC, Yoon JJ, Park HD, Kim SH. Optimization of Batch Dilute-Acid Hydrolysis for Biohydrogen Production from Red Algal Biomass. International Journal of Hydrogen Energy 2013;38(14):6130–6136.

Gonzales RR, Sivagurunathan P, Kim SH. Effect of Severity on Dilute Acid Pretreatment of Lignocellulosic Biomass and the Following Hydrogen Fermentation. International Journal of Hydrogen Energy 2016;41(46):21678–21684.

Dussan KJ, Silva DDV, Moraes EJC, Arruda PV, Felipe MGA. Dilute-Acid Hydrolysis of Cellulose to Glucose from Sugarcane Bagasse. Chemical Engineering Transactions 2014;38:433–438.

Jin F, Zhou Z, Moriya T, Kishida H, Higashijima H, Enomoto H. Controlling Hydrothermal Reaction Pathways to Improve Acetic Acid Production from Carbohydrate Biomass. Environmental Science and Technology 2005;39(6):1893–1902.

Miller GL. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry 1959;31(3):426–428.

Rasmussen H, Sorensen HR, Meyer AS. Formation of Degradation Compounds from Lignocellulosic Biomass in the Biorefinery: Sugar Reaction Mechanisms. Carbohydrate Research 2014;385:45–57.

Kim YS, Jang JY, Park SJ, Um BH. Dilute Sulfuric Acid Fractionation of Korean Food Waste for Ethanol and Lactic Acid Production by Yeast. Waste Management 2018;74:231–240.

Rajan K, Carrier DJ. Effect of Dilute Acid Pretreatment Conditions and Washing on the Production of Inhibitors and on Recovery of Sugars During Wheat Straw Enzymatic Hydrolysis. Biomass and Bioenergy 2014;62:222–227.

Jonsson LJ, Martin C. Pretreatment of lignocellulose : Formation of inhibitory by-Products and Strategies for Minimizing Their Effects. Bioresource Technology 2016;199:103–112.

Zhu S, Huang W, Huang W, Wang K, Chen Q, Wu Y. Pretreatment of Rice Straw for Ethanol Production Dilute Sulfuric Acid and Sulfomethylation Reagent. Applied Energy 2015;154:190–196.

Kumar G, Cheon Hc, Kim Sh. Effects of 5 Hydromethylfurfural Levulinic Acid and Formic Acid Pretreatment by Products of Biomass , on Fermentative H 2 Production from Glucose and Galactose. International Journal of Hydrogen Energy 2014;39(30):16885–16890. 82 Hamzah ET AL.

Md Saiful Islam, Chen Guo CZL. Enhanced Hydrogen and Volatile Fatty acid Production from Sweet Sorghum Stalks by Two-Steps Dark Fermentation with Dilute Acid Treatment in Between. International Journal of Hydrogen Energy 2018;43(2):659–666.




DOI: http://dx.doi.org/10.12962/j20882033.v31i1.5639

Refbacks

  • There are currently no refbacks.


Creative Commons License

IPTEK Journal of Science and Technology by Lembaga Penelitian dan Pengabdian kepada Masyarakat, ITS is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Based on a work at https://iptek.its.ac.id/index.php/jts.